Protein phosphorylation is a ubiquitous and key step in signaling pathways. For flow cytometry staining, the right protocol can help accurately capture these phosphorylated targets for clear-cut data.

In this case study, researchers at the Weizmann Institute of Science in Israel developed and optimized an imaging flow cytometry (IFC) protocol to provide a high-throughput, quantitative and highly ...

If you work in cell biology or immunology, chances are that you're familiar with flow cytometry. This technique measures and analyzes biological cells by suspending them in a stream of fluid and ...

insights from industryNatalie Strickland Global Head of Translational ScienceSynexa Life Sciences In this interview, News Medical speaks with Natalie Strickland, Global Head of Translational Science ...

In this report we propose an outline for the transfection of mtKeima into a cell monolayer, cell preparation and downstream analysis using flow cytometry and associated software, with emphasis on a ...

Flow cytometry is a way to look closely at the features of cells or particles. A sample of blood or tissue goes into a machine called a cytometer. In less than a minute, a computer can analyze ...

Around the same time, Mack Fulwyler, an engineer working at Los Alamos National Laboratory, needed to separate particles, so he drew on existing techniques to create droplets to separate cells from a ...

Human umbilical vein endothelial cells (HUVECs) were seeded at 1 × 10 4 cells per cm 2 in six-well plates. Treatment with TNF-α (2.5, 5 or 10 ng/ml) or lipopolysaccharide (0.5, 1 or 5 μg/ml) treatment ...

The following article is an opinion piece written by Rosa Sottile, PhD. The views and opinions expressed in this article are those of the author and do not necessarily reflect the official position of ...